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Cholesteatoma and hydrocephalus associated to a third ventricle meningioma in a cat Ciência Rural
Wentz,Maria Fernanda; Vargas,Thainã Piccolo; Bianchi,Matheus Viezzer; Fett,Rochana Rodrigues; Lorenzo,Cíntia De; Pavarini,Saulo Petinatti.
ABSTRACT: Meningiomas are neoplasms that commonly involve the central nervous system of cats, while cholesteatomas are nodular granulomatous chronic lesions within the choroid plexus that are rarely reported in cats. This study described a case of cholesteatoma and non-communicating hydrocephalus associated to a third ventricle meningioma in a cat. Clinically, the cat had a 2-year history of behavioral changes, photophobia and motor incoordination. At the necropsy, a tan-brown mass totally occluded the third ventricle, causing a severe dilation of the lateral ventricles (non-communicating hydrocephalus). Microscopically, the mass was composed by a neoplastic proliferation of spindle cells arranged in bundles, containing in the center psammomatous bodies...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Non-communicating hydrocephalus; Immunohistochemistry; Intraventricular tumors; Domestic cats.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001000551
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Choline acetyltransferase detection in normal and denervated electrocyte from Electrophorus electricus (L.) using a Confocal Scanning Optical Microscopy Analysis Anais da ABC (AABC)
NUNES-TAVARES,NILSON; CUNHA-E-SILVA,NARCISA LEAL; HASSÓN-VOLOCH,AÍDA.
Acetylcholine is the neurotransmitter responsible for the transmission of impulses from cholinergic neurons to cells of innervated tissues. Its biosynthesis is catalyzed by the enzyme Choline acetyltransferase that is considered to be a phenotypically specific marker for cholinergic system. It is well known that the regulation of Choline acetyltransferase activity under physiological and pathological conditions is important for development and neuronal activities of cholinergic functions. We observed the distribution of Choline acetyltransferase in sections from the normal and denervated main electric organ sections of Electrophorus electricus (L.) by immunofluorescence using a anti-Choline acetyltransferase antibody. The animals were submitted to a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Choline acetyltransferase; Electrocyte; Denervation; Immunohistochemistry.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000300007
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Chromogenic in situ hybridization compared with other approaches to evaluate HER2/neu status in breast carcinomas BJMBR
Rosa,F.E.; Santos,R.M.; Rogatto,S.R.; Domingues,M.A.C..
Human epidermal growth factor receptor 2 (HER2) has been evaluated in breast cancer patients to identify those most likely to benefit from herceptin-targeted therapy. HER2 amplification, detected in 20-30% of invasive breast tumors, is associated with reduced survival and metastasis. The most frequently used technique for evaluating HER2 protein status as a routine procedure is immunohistochemistry (IHC). HER2 copy number alterations have also been evaluated by fluorescence in situ hybridization (FISH) in moderate immunoexpression (IHC 2+) cases. An alternative procedure to evaluate gene amplification is chromogenic in situhybridization (CISH), which has some advantages over FISH, including the correlation between HER2 status and morphological features....
Tipo: Info:eu-repo/semantics/article Palavras-chave: CISH; Breast cancer; HER2; Immunohistochemistry; FISH.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300207
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Clinical and pathological aspects of multicentric hemangiosarcoma in a Pinscher dog Arq. Bras. Med. Vet. Zootec.
Martins,B.D.C.; Torres,B.B.J.; Rodriguez,A.A.M.; Gamba,C.O.; Cassali,G.D.; Lavalle,G.E.; Martins,G.D.C.; Melo,E.G..
A 3-year-old female Pinscher dog was presented with a history of lameness and pain in hind limbs for one week which acutely progressed to non-ambulatory paraparesis. The dog had been apathetic and anorexic during the six days prior to presentation. The neurological findings were compatible with upper motor neuron signs to the left hind limb due to a spinal cord compressive mass on the twelfth thoracic vertebral body. On the other hand, signs of lower motor neurons to the right hind limb were due to a mass with a pathological fracture in the right proximal femur, compressing the sciatic nerve. A histopathological analysis of all organs revealedthe proliferation of endothelial cells showingin filtrative growth and organization into vascular structures with a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Neuropathy; Hemangiosarcoma; CD-31; Immunohistochemistry.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000200003
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Commentary: Is the Evidence for Localization of Nephrin on the Slit Diaphragm Rigid? International Journal of Morphology
Hipkaeo,Wiphawi; Suzuki,Ryoji; Abe,Hiroshi; Chaisiwamongkol,Kowit; Kondo,Hisatake.
After careful observation of immuno-electron microscopic findings of the renal glomerular podocyte slit so far published by various authors, it should be noted that no convincing evidence is present for localization of nephrin-immunoreactivity in the slit itself, but that nephrin-immunoreactivity is dominantly localized along the membranes of podocyte pedicles from their tip over a substantial extension. In addition, the notion that the slit diaphragm is composed of strands is compatible with the widely distributed view that the slit strands represent rod-shaped nephrin molecules. However, 3-D findings that the diaphragm is composed of not only strands but also membranous elements have recently demonstrated separately by embedment-free section electron...
Tipo: Journal article Palavras-chave: Glomerulus; Slit; Nephrin; Localization; Immunohistochemistry.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400016
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Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver Biocell
Martín,Carlos A.; Surur,José Miguel; García,Marcela N.; Corrons,Félix; Badrán,Amado F..
Variations in DNA synthesis (DNAs) and Nucleolar Organizer Regions (NORs) were studied in the littoral cell population from regenerating liver of C3HS inbred mice standardized for periodicity analysis. Immunohistochemical detection of Bromodeoxyuridine (BrdU) with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections. Tissue samples were obtained every four hours from the 30th to the 54th hours after a partial hepatectomy. The results showed, in both parameters, a gradual increment of the values during the period studied, with highest values (DNAs 107.1 ± 16.1 SE; AgNORs 77.3 ± 3.4 SE) located at 16:00/54 Time of Day / Hours Post- Hepatectomy (TD/HPH), which were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Liver regeneration; Sinusoid littoral cell; Bromodeoxyuridine; AgNOR; Immunohistochemistry.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000100005
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Comparative study of histopathology and immunohistochemistry of indefinite round cell cutaneous tumors and characterization of canine lymphoma Arq. Bras. Med. Vet. Zootec.
Machado,L.H.A.; Palumbo,M.I.P.; Zahn,F.S.; Amorim,R.L.; Farias,M.R.; Werner,J.; Torres Neto,R.; Rodrigues,J.C.; Oliveira,F.C..
With the purpose of shedding light on some doubts in veterinary oncology, the present article intends to compare the results of histopathological and immunohistochemical examinations of unspecific round cell neoplasia, to realize immunophenotyping of canine lymphoma cases, to establish the T or B origin of neoplastic cells, and to determine the degree of proliferation and apoptosis of lymphomas by immunohistochemistry. Of 11 animals presenting immunohistochemical diagnosis of lymphoma, five had been diagnosed as Lymphoma by HE staining of histopathological slides and six had been classified as unspecific round cell neoplasia. All cases submitted to immunohistochemical examination were T-cell lymphomas. There was a positive correlation between cell...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Histopathology; Immunohistochemistry; Round cell neoplasia; Lymphoma.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000100032
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Comparison of blood neoangiogenesis and lymphatic vascularization in colorectal adenomas from patients with and without concomitant colorectal cancer BJMBR
Moreira,L.R.; Schenka,A.A.; Latuf Filho,P.; Lima,C.S.P.; Trevisan,M.A.S.; Vassallo,J..
Blood and lymphatic vessel proliferation is essential for tumor growth and progression. Most colorectal carcinomas develop from adenomas (adenoma-carcinoma sequence) in a process due to accumulation of molecular genetic alterations. About 5% of adenomatous polyps are expected to become malignant, but data on the differential angiogenic patterns of these lesions in patients with and without concomitant cancer are missing. The aim of the present study is to compare the angiogenic and lymphatic patterns of adenomatous polyps from patients with and without sporadic cancer. Thirty adenomatous polyps (15 from patients with another principal malignant lesion, and 15 from patients without cancer) were submitted to immunohistochemical staining for CD105 (marker for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiogenesis; Colorectal adenoma; Colorectal cancer; Immunohistochemistry; CD105; D2-40.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700002
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Comparison of Proliferative Activity of Epithelial Cells between the Follicles of Impacted Teeth International Journal of Morphology
Kömerik,Nurgül; Tufan,Alper; Kapucuoglu,Nilgün.
The aim of this comparative observational study was to compare the proliferative activity of dental follicles surrounding impacted maxillary canines and mandibular third molars. Following extraction, forty follicles of the impacted mandibular third molars and 40 follicles of the impacted maxillary canines were removed. Epithelial cell proliferative activity of these samples was assessed using immunohistochemical labeling for Ki-67, minichromosome maintenance 2 (MCM-2) protein and epithelial growth factor receptor (EGFR). Intensity and extent of Ki-67, MCM-2 and EGFR expressions were evaluated by a scoring formula. The lining epithelium of the maxillary canine follicles had mean scores of 4.65±0.27 for Ki-67, 1.25±0.33 for MCM-2 and 7.30±0.23 for EGFR which...
Tipo: Journal article Palavras-chave: Dental follicle; Ki-67; MCM-2; EGFR; Immunohistochemistry.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400028
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Correlation between chronic inflammation, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum Ciência Rural
Oliveira,Vinícius Vasconcelos Gomes de; Macedo,Simone Regina Barros de; Araújo,Flábio Ribeiro de; Ramos,Carlos Alberto do Nascimento; Ramos,Rafael Antonio Nascimento; Maia,Frederico Celso Lyra; Alves,Leucio Câmara; Silva Junior,Valdemiro Amaro da.
ABSTRACT: The aim of the present study was to investigate the correlations among chronic inflammatory reaction, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum . Animals (n = 10) used in this study were from the Department of Vector Control and Animal Surveillance of the municipality of Caruaru, state of Pernambuco, Brazil. Fragments of the vulva, vagina, cervix, uterine body, uterine horns and ovaries were submitted to histopathological analysis, immunohistochemistry (IHC) and DNA detection of amastigotes by qPCR. Correlations were found between the IHC findings and chronic inflammatory infiltrate related to L. Infantum only in the vulva and vagina; whereas, the same inflammatory reactions...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine visceral leishmaniasis; Genital pathology; Immunohistochemistry; QPCR.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001102029
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Correlative microscopy of Purkinje cells Biocell
Castejón,Orlando J..
The Purkinje cell and their synaptic contacts have been described using (1) light microsocopy, (2) transmission and scanning electron microscopy, and freeze etching technique, (3) conventional and field emission scanning electron microscopy and cryofracture methods, (4) confocal laser scanning microscopy using intravital stain FM64, and (5) immunocytochemical techniques for Synapsin-I, PSD9-5, GluR1 subunit of AMPA receptors, N-cadherin, and CamKII alpha. The outer surface and inner content of plasma membrane, cell organelles, cytoskeleton, nucleus, dendritic and axonal processes have been exposed and analyzed in a three-dimensional view. The intramembrane morphology, in bi- and three-dimensional views, and immunocytochemical labeling of synaptic contacts...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerebellar cells; Synaptic contacts; Electron microscopy; Immunohistochemistry.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100001
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Cyclin D1 expression in prostate carcinoma BJMBR
Pereira,R.A.; Ravinal,R.C.; Costa,R.S.; Lima,M.S.; Tucci,S.; Muglia,V.F.; Dos Reis,R.B.; Silva,G.E.B..
The purpose of this study was to investigate the relationship between cyclin D1 expression and clinicopathological parameters in patients with prostate carcinoma. We assessed cyclin D1 expression by conventional immunohistochemistry in 85 patients who underwent radical prostatectomy for prostate carcinoma and 10 normal prostate tissue samples retrieved from autopsies. We measured nuclear immunostaining in the entire tumor area and based the results on the percentage of positive tumor cells. The preoperative prostate-specific antigen (PSA) level was 8.68±5.16 ng/mL (mean±SD). Cyclin D1 staining was positive (cyclin D1 expression in >5% of tumor cells) in 64 cases (75.4%) and negative (cyclin D1 expression in ≤5% of tumor cells) in 21 cases (including 15...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Prostate cancer; Cyclin D1; Immunohistochemistry.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600515
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Cyclooxygenase-2 expression in epithelial neoplasms and its relevance as a targeted therapy in dogs Ciência Rural
Cunha,Rúbia Monteiro de Castro; Horta,Rodrigo dos Santos; Lavalle,Gleidice Eunice; Araújo,Roberto Baracat.
ABSTRACT: Targeted therapy of neoplasms is an emergent approach in human and veterinary medicine. Cyclooxygenase (COX) is a class of catalytic enzymes related to the formation of inflammatory mediators. COX-2 is expressed constitutively in a few body tissues, but it may be induced in specific pathophysiologic conditions, such as cancer. COX-2 expression in neoplams may be considered a potential predictive factor, due to the possible association of selective COX-2 inhibitors in adjuvant treatments. This scientific communication has the objective to report COX-2 expression in seven neoplasms of dogs and the usage of adjuvant treatment with COX-2 selective inhibitors as an effective and feasible option in cancer treatment.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cancer; Carcinoma; Immunohistochemistry; Antineoplastic drugs..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000601050
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Death-Associated Protein Kinase is Underexpressed in High-Grade Oral Squamous Cell Carcinoma International Journal of Morphology
Meneses,Ismário Silva de; Souza,Rafael Reis de; Jeraldo,Verónica de Lourdes Sierpe; Cavalcante,Danielle Rodrigues Ribeiro; Reis,Francisco Prado; Albuquerque Júnior,Ricardo Luiz Cavalcanti de.
An immunohistochemical analysis of 40 cases of oral squamous cell carcinoma was performed to evaluate the relationship between the expression pattern of death-associated protein kinase (DAPk) positive cells with the histological malignancy grading of these lesions. According to our results, eleven cases (27.5%) were high-grade malignancy tumours and 29 (72.5%) were low-grade ones. We found that 92.86% of the low-grade tumours were positive to anti-DAP kinase antibody whereas only 7.14% of the high-grade tumours presented positivity, and this difference was statistically significant (p<0.01). Sixteen (55.2%) of the low-grade carcinomas exhibited moderate immunoreactivity whereas ten cases (34.5%) showed weak staining and three cases (10.3%) were negative...
Tipo: Journal article Palavras-chave: Immunohistochemistry; DAP kinase; Apoptosis; Squamous cell carcinoma.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200044
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Detection of CD4+ and CD8+ lymphocytes in the intestine of broiler chicks treated with Lactobacillus spp. and challenged with Salmonella enterica serovar Enteritidis Rev. Bras. Ciênc. Avic.
Noujaim,JC; Andreatti Filho,RL; Lima,ET de; Okamoto,AS.
The expression of immune response as a leukocytic infiltrate by CD4+ and CD8+ cells in the epithelium and in the intestinal lamina propria of chicks fed Lactobacillus spp or cecal microflora (CM) and experimentally challenged or not with Salmonella enterica serovar Enteritidis (SE) was studied using immunohistochemistry. Three hundred and twenty day-of-hatch broiler chicks were divided into four groups of 80 birds each and orally received L. reuteri, L. salivarius, L. acidophilus, or CM. Each group was subdivided into four subgroups of 20 birds each, classified as follows: a subgroup did not receive any oral treatment (negative control), subgroup treated with L. spp or CM, subgroup treated with L. spp or CM and challenged with SE, and subgroup only...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken; Immunohistochemistry; Lymphocytes CD4+ and CD8+; Probiotic; Salmonella.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000300008
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Detection of human parvovirus B19 in a patient with hepatitis BJMBR
Pinho,J.R.R.; Alves,V.A.F.; Vieira,A.F.; Moralez,M.O.S.; Fonseca,L.E.P.; Guz,B.; Wakamatsu,A.; Cançado,E.L.R.; Carrilho,F.J.; da Silva,L.C.; Bernardini,A.P.; Durigon,E.L..
Parvovirus B19 has been associated by some investigators with cases of severe hepatitis. The aim of the present study was to determine the presence of active parvovirus B19 infection among 129 Brazilian patients with non-A-E hepatitis. The patients were assayed for antibodies against parvovirus B19, IgM class, by ELISA. In IgM-positive cases, parvovirus B19 DNA was assayed by PCR in serum and liver tissue and parvovirus VP1 antigen in liver tissue was assayed by immunohistochemistry. Antibodies against parvovirus B19, IgM class, were detected in 3 (2.3%) of 129 patients with non-A-E hepatitis. Previous surgery and blood transfusions were reported by these 3 patients. One patient was a 56-year-old female with severe hepatitis, with antimitochondrial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Parvovirus B19; Hepatitis non-A-E; Autoimmune hepatitis; Polymerase chain reaction; Immunohistochemistry.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900005
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Differential Effect of the Route of Inoculation of Rabies Virus on NeuN Immunoreactivity in the Cerebral Cortex of Mice International Journal of Morphology
Rengifo,Aura Caterine; Umbarila,Vanessa Jazmin; Garzón,Mary Janeth; Torres-Fernández,Orlando.
Rabies is a lethal disease caused by a neurotropic virus that produces inconspicuous morphological changes hardly observable with conventional histopathology. The fatal outcome caused by rabies could be attributed to specific biochemical changes that severely impact neuronal function. The neuronal nuclear protein (NeuN) has become a widely used neuronal marker for the research and the histopathological diagnosis of nervous system diseases. To evaluate the distribution of the protein NeuN in the motor cortex of normal and rabies-infected mice adult ICR mice were inoculated with rabies virus either intramuscularly or intracerebrally. Rabies-infected mice were sacrificed at the terminal stage of the disease. Control mice were also euthanized at the same age....
Tipo: Journal article Palavras-chave: Rabies; NeuN; Cerebral cortex; Mouse; Immunohistochemistry; Neurons.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400031
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Differential patterns of myosin Va expression during the ontogenesis of the rat hippocampus BJMBR
Brinn,L.S.; Pereira Leite,J.; Larson,R.E.; Martins,A.R..
Myosin Va is an actin-based, processive molecular motor protein highly enriched in the nervous tissue of vertebrates. It has been associated with processes of cellular motility, which include organelle transport and neurite outgrowth. The in vivo expression of myosin Va protein in the developing nervous system of mammals has not yet been reported. We describe here the immunolocalization of myosin Va in the developing rat hippocampus. Coronal sections of the embryonic and postnatal rat hippocampus were probed with an affinity-purified, polyclonal anti-myosin Va antibody. Myosin Va was localized in the cytoplasm of granule cells in the dentate gyrus and of pyramidal cells in Ammon's horn formation. Myosin Va expression changed during development, being...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Myosin Va; Growth and development; Central nervous system; Hippocampus; Rat; Immunohistochemistry.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900012
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Distribution and Morphology of Ghrelin-Immunopositive Cells in the Thymus of the African Ostrich International Journal of Morphology
Wang,Jia-xiang; Li,Peng; Zhang,Xiao-ting; Ye,Li-xun.
Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, has been detected in the thymus of multiple vertebrates. However, little is known about its distribution in the thymus of the African ostrich. In this study, we evaluated the distribution and morphological characteristics of ghrelin-producing cells in the thymus of the African ostrich. Our results revealed that the thymus consists of a capsule and a parenchyma, which comprises the cortex and medulla. Compared to the cortex, the medulla had a fewer number of thymocytes and a greater number of epithelial cells. Additionally, three thymic corpuscles were identified. Ghrelin-immunopositive (ghrelin-ip) cells were detected both in the cortex and medulla of the African ostrich thymus,...
Tipo: Journal article Palavras-chave: African ostrich; Ghrelin; Thymus; Immunohistochemistry.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200026
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Distribution of biglycan and decorin in rat dental tissue BJMBR
Tenório,D.M.H.; Santos,M.F.; Zorn,T.M.T..
Biglycan and decorin are small leucine-rich proteoglycans that play several biological and structural roles in different tissues and organs. Several reports have indicated that biglycan participates in odontoblast and ameloblast differentiation and in the calcification process. In the present study we show that the expression of biglycan changes from within the ameloblasts and odontoblasts to the extracellular space according to the stage of animal development. In predentin and in the pulp space, however, biglycan was continually expressed throughout the period of investigation. In contrast, decorin was absent in odontoblasts and in ameloblasts and was exclusively expressed in predentin throughout the period of observation. In young rats, however, decorin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Decorin; Biglycan; Odontoblasts; Predentin; Immunohistochemistry.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800012
Registros recuperados: 124
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